Abstract: In the present review two versatile analytical technique HPLC and UPLC discussed thoroughly for their unique properties, working and detectors which makes them very valuable in the drug and pharmaceutical industry. Here discussion starts from the origin of chromatography, types of chromatography techniques. It involves the intricate details of the functioning of HPLC and UPLC and how they are different from each other.

Keywords: Chromatography, HPLC, UPLC, Detectors, Separation techniques.

| DOI: 10.17148/IARJSET.2020.7502