Abstract: Millets are used for their rich protein content. These days there are two types of millets that are present in the market one is organic and the other type is non-organically grown millets. In the present work analysis of the difference in protein composition of organic and non-organically grown Sorghum and Barnyard millets. Centrifugation method was used to extract 4 types of proteins (ie., Storage proteins) namely Albumin, Globulin, Prolamins and Glutenin. Spectroscopic method (Microplate method) and SDS Gel Electrophoresis methods were applied to determine the protein composition using advanced application like Color grab ‘Software J’, Curve fit tools to estimate the proteins. As per the results obtained by our observations there was only four fraction difference between them which is considered as negligible in the protein composition, but in market there is double the price for Organic millets compared to Non-organic millets.

| DOI: 10.17148/IARJSET.2021.81025