ANTIMICROBIC, CYTOTOXICITY AND DNA CLEAVAGE EVALUATION OF SILVER NANOPARTICLES

Abstract: The present set-up of bio-medical applications stresses on the cost effectiveness and eco-friendly nature of Nanoparticles which are very well answered by silver nanoparticles making it a front runner towards the biomedical investigation and assay. For this purpose, in this study, we aimed to synthesize the silver nanoparticles (AgNps) using chemical reduction method, a Partial Green Synthesis involving Ascorbic acid a form of Vitamin-C. The Synthesized AgNps were characterized using UV-Visible Spectroscopy, X-ray diffraction (XRD) and TEM. The antimicrobic studies included the bactericidal and fungistatic evaluation of AgNps on bacterial strains S. aureus, S. mutans and the fungal strains of A. niger, C. albicans. Norfloxacin a standard antibacterial drug and Griseofulvin a well- known antifungal drug was used as standards for the study. Cytotoxic evaluation was conceded using protocol of Meyer et al (brine shrimp bioassay). The DNA dissociation activity of synthesized AgNPs was studied. The AgNPs were found to be more susceptible towards the bacterial strains as compared to the fungal strains.

Keywords: Silver nanoparticles (AgNps), Cytoxicity, DNA cleavage, Norfloxacin, Griseofulvin.