Abstract: Carbohydrate, Protein, Flavonoid and Phenol are important component of fruits and vegetable. for knowing its actual amount present in different fruits and vegetable sample we take 10 different fruits and vegetables sample . The samples are collected from vegetables and fruit market of Patna . All the above samples are consumed by the people, and biomolecules present in it have major importance to life, and many biological aspects dealing vital in science. The total protein content was determined using the Lowry method. The total protein content was also measured by spectrophotometrically. The estimation showed that the Malus domestica (Apple) contain highest protein content among all other selected samples, it records an optical density of 0.214 at 750 nm which indicate that it contains total 516 µg/ml of protein content. The total carbohydrates content was determined using by DNSA method. The total carbohydrates content was also measured by spectrophotometrically. The estimation showed that Musa acuminate (banana) contain highest carbohydrates among all other selected sample, it recorded an optical density of 1.649 at 540 nm which means banana contain 555.793 µg of carbohydrate content in per ml of sample extract. The total Flavonoid content was determined using by aluminium chloride colorimetric method. The estimation showed that the Malus domestica( Apple) contains highest flavonoid content, it record an absorbance of 0.214 at 510 nm and contain 516.75 µg/ml of flavonoid content. The Phenolic content estimation is done by Folin–Ciocalteu method. The estimation showed that the Malus domestica( Apple) record an absorbance of 0.370 at 650 nm, and contains 44.30 µg/ml of phenolic content. It is the highest phenol containing sample among all other sample.

Keywords: protein, carbohydrates, flavonoids, phenol

| DOI: 10.17148/IARJSET.2022.9544