Abstract: The main purpose of present study was detection of variations of somaclones in the banana cv. Grand Naine by using simple sequence repeats (SSR) molecular marker technique. Matured leaves that are free from damage caused by pests or disease were collected in brown covers from different field places of Bengaluru, Karnataka. Isolation of genomic DNA was carried out by cetyl trimethyl ammonium bromide. The integrity and purity of DNA and visualization of amplified products was done by electrophoresis and quantified. PCR amplification of DNA from banana somaclones using SSR markers were carried out using Taq DNA polymerase and short stretch of oligonucleotides (primers), which are specific to the DNA to be amplified. Then the SSR, markers are used to assess the genetic variation in somaclonal variants of banana cv. Grand Naine. Out of 78 SSR primers screened to detect polymorphism, 11 primers showed polymorphism and 45 SSR primers showed only monomorphic bands. In conclusion, the potential of molecular markers has been used for testing the genetic fidelity of micropropagated cv. Grand Naine (AAA) plants in the fields. Molecular marker like SSR were used to identify the genetic variations that has resulted in banana cv. Grand Naine. SSR markers tried clearly showed polymorphism between the off types and the normal banana plants. In conclusion, overall 17% polymorphism was observed in variants indicating the level of genetic change.

Keyword: Molecular markers, Polymorphism, SSR, Grand naine (AAA)

| DOI: 10.17148/IARJSET.2022.9427