Abstract : The crude extraction of Cleome gynandra L. leaf and stem obtained in different solvents like methanol, ethanol and water was subjected to preliminary phytochemical analysis and the antibacterial screening against the gram-negative bacteria. Phytochemical study of both stem and leaf extracts shows the presence of secondary metabolites like Alkaloid, Cardiac glycosides, Flavonoids, Phlobatannins, Amino acids and Proteins which were predominantly present in all tested solvent extracts, followed by Saponins were present only in the methanol and water extract of both stem and leaf. Among all the three tested solvent of stem and leaf extract shows the various degree of significant antibacterial activity against Escherichia coli and Pseudomonas aeruginosa. Methanol extract of leaf shows the maximum inhibitory activity against E. coli and ethanol extract of both stem and leaf shows the similar inhibitory action against both tested bacteria. Water extract of stem and leaf shows the minimum inhibition with reference to the standard (Chloramphenicol).

Keywords- Phytochemical analysis, Antibacterial activity, Cleome gynandra L.

| DOI: 10.17148/IARJSET.2021.81227