Abstract: The present study is aimed to investigate the bioactive compounds present in the tested plant by using Thin Layer Chromatography (TLC). Thevetia peruviana (Pers.) K. Schum is one of the important medicinal as well as ornamental plant belongs to the Dogbane family Apocynaceae. It is commonly known as yellow oleander. The plant is popular in traditional medicine especially for treatment of heart problems. The leaves are emetic and purgative. Leaf decoction is given to prevent conception. Thin layer chromatographic analysis reveals the presence of different bioactive compounds like alkaloids, flavanoids, cardiac glycosides, phenolic compounds, phytosterols and tannins, which are mainly contributed to medicinal utility of the plant, hence, Thevetia White ethanolic leaf extract can be used for drug preparation in pharmaceutical industries.

Keywords: Thin Layer Chromatography, cardiac glycosides, phytochemical analysis, Thevetia peruviana (Pers.) K. Schum.

| DOI: 10.17148/IARJSET.2021.81107